A pathogen peptidoglycan scaffold coated with artificial biomembrane promotes broad resistance to bacterial infections by dynamically reprogramming macrophage metabolism.

The data includes LM@pBLP Characterization experiments, particle size and potential, transmission electron microscopy, Fourier transform infrared spectroscopy, flow cytometry, safety experiments. LM@pBLP Induced rapid protection, broad-spectrum protection, long-lasting protection, in vitro macrophage experiments, mouse in vivo experiments, and in vitro macrophage experiments. Measure the expression levels of cytokines and training immune markers using qPCR. Measure the concentrations of lactate, glucose, ROS, and NO in the supernatant and serum of mouse cells using a detection kit. Survival rate and weight changes of mice.本数据集包含LM@pBLP表征实验、粒径与电位测试、透射电子显微镜（Transmission Electron Microscopy）、傅里叶变换红外光谱（Fourier Transform Infrared Spectroscopy）、流式细胞术（Flow Cytometry）及安全性实验。LM@pBLP可诱导快速防护、广谱防护与长效防护，相关实验涵盖体外巨噬细胞实验、小鼠体内实验，以及体外巨噬细胞实验。采用qPCR（quantitative Polymerase Chain Reaction）检测细胞因子与训练免疫标志物的表达水平；借助检测试剂盒测定小鼠细胞上清液与血清中乳酸、葡萄糖、活性氧（Reactive Oxygen Species, ROS）、一氧化氮（Nitric Oxide, NO）的浓度；同时记录小鼠的存活率与体重变化。