Supplementary material (raw data) for Fig. 2 in "Precision and accuracy of single-molecule FRET measurements – a multi-laboratory benchmark study" to be published with Nature Methods The confocal da
represents the lifetimes of the donor-only decay and represents the fractional contribution of each component. represents the FRET efficiency for each component as defined in Equations 9–13. All th
This data set is a collection of usALEX smFRET burst data measured for the lacCONS promoter DNA labeled at the transcription bubble region (in initiation). Each measurement follows the results in diff