Global gene transcription analysis in response to changes in chromosome supercoiling in Streptomyces coelicolor. Global gene transcription analysis in response to changes in chromosome supercoiling in Streptomyces coelicolor

Here, we address the transcriptional response to both novobiocin-induced rapid chromosome relaxation or long-term topological imbalance, both increased and decreased supercoiling, in environmental antibiotic-producing bacteria belonging to the Streptomyces genus. Overall design: RNA was extracted from vegetative hyphae of Streptomyces coelicolor growing for 18h in liquid YEME/TSB medium (30 mL). To induce supercoiling changes S. coelicolor culture was treated with novobiocin in final concentration 10 g/ml for 10 min (rapid chromosome relaxation). For long-term exposure to topological imbalance we used PS04 stain in which TopA level is controlled by thiostrepton addition (long-term high and low DNA supercoling in not induced and induced PS04 strain, respectively)