BXSB_mice_IgG_glycosylation_batch2

Batch 2. The folder Batch2 contains mzXML files obtained in a liquid chromatography-mass spectrometry (LC-MS) analysis of Fc-linked IgG N-glycosylation profiles of mouse immunoglobulin G in a BXSB mice cohort, batch 2. First part of batch1 can be found in the following dataset: 10.17632/jygxpd7g7c.1 Second part of batch1 can be found in the following dataset: http://dx.doi.org/10.17632/kjfw7cwnxf.1 Male BXSB mice spontaneously develop an autoimmune disorder similar to systemic lupus erethematousus (SLE) which involves production of autoreactive antibodies. We have isolated IgG from serum sampled at 10 and 18 weeks of age in a cohort of sex-matched BXS mice, treated either with anti-CD20 specific antibody that transiently depletes B-cells during the pre-phase of the disease or with an isotype antibody (control). IgG was digested with trypsin, the glycopeptides desalted and enriched by reverse-phase solid phase extraction and analyzed on an LC-MS system. We observed higher abundance of sialylated IgG1 and IgG2b/IgG2c glycoforms in the male mice treated with CD20-specific antibody as compared to isotype-treated male mice, while no major changes were observed in galactosylated IgG glycoforms between isotype and CD20-specific antibody treated male mice. Information about glycoforms observed in the samples and corresponding m/z values of analytes can be found in the excel sheet: Supplementary Table S1.xlsx Description of the samples could be found in the following excel sheet: Supplementary Table S2.xlsx批次2。 本批次2文件夹包含了针对BXSB小鼠队列的小鼠免疫球蛋白G（immunoglobulin G, IgG）的Fc段连接型N-糖基化谱进行液相色谱-质谱联用（liquid chromatography-mass spectrometry, LC-MS）分析所得的mzXML文件。 批次1的第一部分可在如下数据集获取：10.17632/jygxpd7g7c.1 批次1的第二部分可在如下数据集获取：http://dx.doi.org/10.17632/kjfw7cwnxf.1 雄性BXSB小鼠可自发产生与系统性红斑狼疮（systemic lupus erythematosus, SLE）相似的自身免疫性疾病，该疾病伴随自身反应性抗体的产生。 本研究从性别匹配的BXS小鼠队列中采集了10周龄和18周龄的血清，并从中分离IgG；受试小鼠分别接受可在疾病前驱期暂时性耗竭B细胞的抗CD20特异性抗体治疗，或同型对照抗体治疗。将分离得到的IgG用胰蛋白酶进行酶解，随后对糖肽进行脱盐处理，并通过反相固相萃取进行富集，最后采用LC-MS系统进行分析。 本研究观察到，相较于接受同型对照抗体治疗的雄性小鼠，接受抗CD20特异性抗体治疗的雄性小鼠体内，唾液酸化IgG1及IgG2b/IgG2c糖型的丰度显著升高；而两组小鼠的半乳糖基化IgG糖型未出现明显变化。 样本中检测到的糖型信息及对应分析物的质荷比（m/z）可参见Excel表格：Supplementary Table S1.xlsx 样本的详细描述可参见如下Excel表格：Supplementary Table S2.xlsx