RNA-seq using human SH-SY5Y neuroblastoma cells used for SILAC experiment

To measure translational efficiency in FMRP depletion, we purified RNAs from either wild-type or FMR1-knockout (FMR1-KO) SH-SY5Y cells generated for SILAC coupled to LC-MS/MS analysis and performed RNA-seq to quantitate mRNA abundance to normalize their protein abundance. RNA profiles of wild-type (n=6) and FMR1-knockout SH-SY5Y cells (n=6) culturing in either SILAC heavy (containing 13C6,15N4 L-arginine and 13C6,15N2 L-lysine) or light (containing 12C6,14N4 L-arginine and 12C6 L-lysine) medium.