Targeted differentiation of regional ventral neuroprogenitors and related neuronal subtypes from human pluripotent stem cells
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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Purpose: To find the mechanism for the differences in cell fates between the EB (embryoid body) and AD (adherent) culture conditionsMethods: hESCs were cultured in different methods, and RNA-seq were generated , in triplicate, using Illumina GAIIx. The sequence reads were analyzed with Weighted gene correlation network analysis (WGCNA) and Gene Ontology (GO) and TopHat followed by Cufflinks. qRTâPCR validation was performed using TaqMan and SYBR Green assaysResults: We totally found roughly 600 genes which were specifically enriched in both day 4 EB cells and day 4 EB cells treated with SB431542 and LDN193189. Meanwhile, there were around 1000 genes which showed significant higher expression in AD cellsConclusions: complex intracellular cell contexts and extracellular components work together and define the distinct differentiation potencies of the AD and EB cells upon SHH patterning.
提供机构：
Tongji University
创建时间：
2022-02-20
