ATAC-seq analysis in Rpsa-iKO RAW264.7 cells after HSV-1 infection

After HSV-1 infection, ribosomal protein RPSA can recognize viral DNA and promotes the expression of proinflammatory cytokines. Through ATAC-seq, we found that chromatin accessibility around transcription start sites (TSS) of proinflammatory cytokinse in HSV-1 infected Rpsa-iKO RAW264.7 was significantly reduced We constructed Rpsa induced konckout RAW264.7 cell lines and performed Assay for Transposase Accessible Chromatin with high throughput sequencing in WT and Rpsa-iKO RAW264.7 cells infected with or without HSV-1.