Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism [1]

IgA+ Plasma Cells were sort-purified from the small intestinal lamina prorpia of mice with a B cell lineage-intrinsic deletion of Arntl (Mb1 Cre+/- x Arntl fl/fl) or Cre negative littermate controls (also deisgnated WT and KO), at two Zeitgeber time points (ZT0 and ZT12). RNA extracted from these samples was subjected to bulk RNA seq to identify time of day differences and to compare role of Arntl expression in this context. Each sample represents IgA+ PC sort-purified from the small intestine of one indivisual animal. Main comparisons are transcriptional signatueres at ZT 0 vs ZT12 (time of day) and in WT (Mb1 Cre-/- x Arntl fl/fl) vs KO (Mb1Cre+/- x Arntl fl/fl) cells.