Study on Salt Tolerance Mechanism via Illumina-based RNA-seq in Chrysanthemum Roots

The samples were respectively from three replicates of the salt stress treatment and the control, then we obtained six cDNA libraries. In total, 983,827 transcripts and 469,059 unigenes were constructed. We got a total of 75,070 differentially expressed genes (DEGs), of which 48,965 were up-regulated and the other 26,105 were down regulated under the salt treatment. Overall design: We used the chrysanthemum “jinba” as the main material and the young seedling was breed for 30 days under the circumstance of 23±2?, photoperiod of 16 hours, respectively moisty of 75%. During the period, the sample was watering controlled for 2 days and mildly salt stressed by salt water with a concentration of 100mM while the controlling sample is watered by purified water. The samples were collected from the root of white seedlings after 24 hours from processing. roots mRNA profiles of the development were generated by deep sequencing, in triplicate, using Illumina HiSeq.