Human Protein Atlas - Blood Atlas

The Blood Atlas contains single cell type information on genome-wide RNA expression profiles of human protein-coding genes covering various B- and T-cells, monocytes, granulocytes and dendritic cells. The single cell transcriptomics analysis covers 18 cell types isolated with cell sorting followed by RNA-seq analysis. In addition, an analysis of the “human secretome” is presented including annotation of the genes predicted to be actively secreted to human blood, as well as the annotation of proteins predicted to be secreted to other parts of the human body, such as the gastric tract and local compartments. An analysis of the proteins detected in human blood is also presented with an estimation of the respective protein concentrations determined either with mass spectrometry-based proteomics or antibody-based immune assays.

《血液图谱》收录了涵盖多种B细胞、T细胞、单核细胞、粒细胞和树突状细胞的蛋白质编码基因的全基因组RNA表达谱的单细胞类型信息。单细胞转录组学分析覆盖了通过细胞分选获得的18种细胞类型，并随后进行RNA测序分析。此外，还呈现了对“人类分泌组”的分析，包括预测为活跃分泌至人体血液的基因的注释，以及预测为分泌至人体其他部位的蛋白质（如胃部及局部腔室）的注释。同时，还展示了检测到的人体血液中的蛋白质分析，并估计了相应的蛋白质浓度，这些浓度是通过基于质谱的蛋白质组学或基于抗体的免疫分析确定的。

搜集汇总

背景与挑战

背景概述

Human Protein Atlas - Blood Atlas数据集提供了人类蛋白质编码基因在多种血液细胞类型中的单细胞转录组分析，包括B细胞、T细胞、单核细胞等18种细胞类型。此外，该数据集还包含对人类分泌组和血液中检测到的蛋白质的分析，包括其浓度估计。