Gene expression changes elicited by PCN and LCA in primary rat hepatocytes.

Quadruplicate cultures of primary rate hepatocytes were exposed to PCN or LCA at the indicated concentrations for 48 hours. RNA was extracted and gene expression monitored using Affymetrix rat 230 v2 microarrays. Statistical Analysis of Microarrays (SAM) analysis was undertaken to identify statistically significant gene expression changes (Dose vs medium). False Discovery Rate (FDR) was manually set to provide sufficient gene changes, while limiting the number of predicted false positives. Numbers of genes altered under each condition are presented for the stated FDR, along with the predicted number of false positives within each group.