TG2 peptide substrates identified by nano-LC MS/MS.

Glutamine residues targeted by TG2 are given in bold.DA, deamidationThe 9mer core region of T-cell epitopes are underlined.“x” indicates I or L.anumber of protein entries in database.bnot possible to determine which Q residue is transamidated (shown in bold and italic).cnot possible to determine which Q residue is deamidated and which is transamidated.dnot possible to determine whether Q2 or Q4 is targeted; however, this sequence has previously been shown to be targeted at Q4 [17]. The same Q residue is expected to be targeted in peptides #12–17.eidentified in a sample incubated with TG2 for one minute.ftwo possible 9mer binding registers to HLA-DQ2.5.