HSV-1 DNA replication does not decrease H3.1 mobilization in U2OS cells.

(A) Average normalized levels of free GFP-H3.1 relative to untreated mock-infected cells. U2OS cells were transfected with plasmids expressing GFP-H3.1. Transfected cells were mock-infected or infected with 6 PFU/cell of strain KOS in the presence of 400 µg/ml PAA (PAA KOS) or no drug (KOS). Mobilization of GFP-H3.1 was examined from 4 to 5 (4 hpi) or 7 to 8 (7 hpi) hpi by FRAP; error bars, SEM; dashed line, average normalized level of free GFP-H3.1 in mock-infected cells. (B) Average initial rates of normalized fluorescence recovery of GFP-H3.1 relative to untreated mock-infected cells; error bars, SEM; dashed line, the average initial rate of normalized fluorescence recovery in mock-infected cells. KOS data re-plotted from Figure 8 for comparison. **, P<0.01; n.s., not significant.