Overview of protein identifications and quantifications.

*target protein (ng) detected on column/total amount protein (ng) detected on column. **excluding top 1% percentile. A first round of LC-MS analysis compared FACS-purified pancreatic alpha and beta cells (n = 3) to whole liver and brain tissue pools. A second independent analytical round compared rat beta cells that were sorted for their metabolic responsiveness (glucose-induced NAD(P)H) shortly after their isolation. ‘High’ and ‘low beta’ indicate respectively beta cells with higher or lower metabolic glucose responsiveness, as reflected by their level of 7.5 mM glucose-stimulated NAD(P)H production (See Fig. S2, Methods). The table shows number of proteins identified (above limit of detection), quantified (above limit of quantification) and general quantitative properties of the proteomes.