DNA unmethylome profiling by covalent capture of CpG sites (microarray). Homo sapiens

Here we present an approach for the mapping of unmethylated DNA. Our technique is based on DNA methyltransferase-directed transfer of activated groups (mTAG) and covalent biotin tagging of unmodified CpG sites followed by affinity enrichment and interogation on tiling microarrays or next generation sequencing. Overall design: mapping of unmethylated DNA