Effects of 20 TDH3 promoter alleles at HO and native TDH3 loci

Comparing the effects of 20 TDH3 promoter alleles driving expression of YFP at the HO locus or driving expression of TDH3-YFP fusion at the native TDH3 locus.
Conclusion:
Absolute effect sizes of mutations on average activity or variability of activity of TDH3 promoter are slightly different between HO locus and native TDH3 locus, but the effects of mutations are strongly correlated between these two loci. Therefore, effects of mutations on TDH3 expression can be directly inferred from the activity of the promoter measured at the HO locus. Each plate contains 20 replicate populations of a wild type strain homogeneously distributed on the plate. These controls are used to correct for technical variation between flow cytometry runs. In addition, each plate contains two replicate populations of a non-fluorescent strain used to correct for autofluorescence.