16S rRNA amplicon sequencing of the human vaginal microbiome

The purpose of this study is to investigate the composition and diversity of the vaginal microbiota in women with vaginitis using 16S rRNA gene amplicon sequencing. Vaginal swab samples were collected from 24 participants. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified and sequenced on the Illumina NovaSeq 6000 platform. The resulting sequences were analyzed to determine the taxonomic profiles, community structure, and relative abundances of bacterial populations. This project aims to provide insights into the performance of the vaginal microbiome under different treatment conditions and to offer new perspectives for clinical treatment.