Single-nucleus mRNA-sequencing reveals dynamics of lipogenic and thermogenic adipocyte populations in murine brown adipose tissue in response to cold exposure

Brown adipocyte-specific ChREBP knockout were generated by crossing mice with a floxed exon1a in the Mlxipl gene with Ucp1-Cre mice. These ChREBPflox/flox Ucp1-Cre+ (Cre+) mice and ChREBP-expressing control (Cre-) mice were studied under various conditions of BAT activation. Housing at room temperature (22?C, RT) was chosen as a control condition when BAT is mildly activated. Cold-exposure (6?C) was carried out for 1 day (acute cold ), as an early stage when proliferative processes of BAT adaptation are not yet taking place, or for 10 days (chronic cold) when BAT is fully expanded.