The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes (Omni-ATAC-seq II)

Human telomerase reverse transcriptase (hTERT)-Immortalized cell line (T-HF) with or without DOX-induced KD of SSPR1 and SPT6 were infected with wild-type simplex virus 1 (HSV-1) strain F or with ICP22-null mutant at a multiplicity of infection (MOI) of 10. Omni-ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Corces, M., Trevino, A., Hamilton, E. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14, 959–962 (2017). https://doi.org/10.1038/nmeth.4396 Overall design: T-HFs were treated with DOX for 72h prior to infection to induce knock down of either SPT6 or SSRP1. Cells were infected with HSV-1 strain F or ICP22-null mutant for 8 hours. PAA was used at 350 µg/mL during the course of infection. Omni-ATAC-seq samples were prepared at the indicated times of infection. Two independent biological replicates were analysed.