Intra-tetrad mating patterns detected in <i>S. cerevisiae</i>.

Single tetrads derived from EAY2771 (relevant genotype ars314::kanMX MATa/ars314::natMX MATalpha) were germinated on YPD media to form single colonies. These colonies were then restreaked to single cells on YPD media. Two independent diploid colonies were isolated either directly from the single cell streak of each germinated tetrad colony or by microdissection of unbudded cells present in a germinated tetrad colony. The two diploid colonies were phenotyped for resistance to G418 and nourseothricin. Colonies that showed resistance to both antibiotics were categorized as inter-spore maters, while those resistant to only one antibiotic were categorized as self-maters. Multiple refers to events where one of the two diploids isolated from a single tetrad was an inter-spore mater and the other a self-mater.