Diurnal ChIP-Seq for Petunia hybrida

To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, cross-linked chromatin from corolla in the morning (7AM) and evening (7PM) was immunoprecipitated with antibodies against 4 histone marks associated with trancriptional activity to determine islands enriched for the marks in morning and evening. Input or immunoprecipitated chromatin from formaldehyde cross-linked corolla tissue was used for library preparation and sequencing, at two time points, with two biological replicates for each ChIP (three input replicates)