Identification and characterization analysis of the different genes in primary mouse microglia response to ACT-PARP14 using RNA-seq.

Poly(ADP-ribose) polymerases (PARPs) modify target proteins with ADP-ribose by using nicotinamide adenine dinucleotide as a substrate, and contribute to various signaling pathways. PARP14 is the largest member of PARP superfamily. Whole RNA-sequencing analysis was performed and analyzed by Lc. Biotech Co., Ltd. (Hangzhou, China). Primary mouse microglia from the Con + ACT-Con group, Con + ACT-PARP14 group, OGD + ACT-Con group, and OGD + ACT-Con group were collected in TRIzol. Total RNAs were extracted from Trizol. UMI technology was used to label each sequence fragment with sequence tags, which minimized the interference of duplication caused by PCR amplification on the quantitative accuracy of the transcriptome. RNA sequencing reads were aligned to the mouse genome (GRCh37/hg19) using the software Hisat2 (2.0.4). Transcript abundance was evaluated by calculating fragments per kilo base of exon per million fragments mapped (FPKM). Examination of different genens in ACT-PARP14-treated or ACT-Con-treated primary mouse microglia by RNA-seq. All data were obtained from 2 independent experiments.