MOESM4 of CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry

Additional file 4: Table S2. Summary of analyses of CRISPR/Cas and wild-type ‘Calypso’ and ‘Hawaii 4’ shoot lines and samples. Sample entries for shoot lines/shoots/samples are pattern and colour coded: solid green, pale green or pale brown shading = green, pale green or off-white tissue, respectively; mottled shading = variegated tissue; no shading = white tissue. Results of PCR screening for presence of T-DNA Nos-Kan cassette and sgRNA insertion are indicated by ‘+’ (amplification of expected product) or ‘-’ (no product). Sequence results obtained for TAIL PCR reactions are indicated by ‘+’; ‘---’ = not done; F - failed sequence analysis. % wild type (WT) reads are shown for target site amplicon sequences: bold font for % WT reads and asterisks highlight results which may be unexpected in relation to observed phenotype. Mutant sequence variant type and putative allele status are shown for each sample.