Use of CGH microarrays to identify strain variable genomic regions in C. botulinum ATCC 3502

Comparative genomic hybridization microarrays featuring overlapping probes spanning the entire C. botulinum type A1 strain ATCC 3502 genome were used to identify regions whose presence are variable among a diverse panel of type A C. botulinum strains. For each hybridization, the reference strain (C. botulinum ATCC 3502) was labeled with Cy5 and test strains were labeled with Cy3.