Tandem-affinity purification of OOPS-1 and SPE-11 and identification of phosphorylation sites on OOPS-1 and SPE-11

Fertilization triggers the completion of female meiosis and launches the oocyte-to-embryo transition. C. elegans spe-11 is one of the few known paternal-effect embryonic lethal genes. We report that the sperm protein, SPE-11, forms a complex with an oocyte protein, OOPS-1 (Oocyte Partner of SPE-11) at fertilization, and that the protein complex is required for the completion of meiosis, the block to polyspermy, and eggshell formation. Tandem-affinity purification and mass spectrometry from C. elegans extracts was used to establish that OOPS-1 and SPE-11 associate. This supplemental dataset provides the mass spectrometry data supporting the finding that OOPS-1 and SPE-11 associate in C. elegans extracts. These data were analyzed and are summarized in Table 3, Table S1, and Table S2 of the manuscript. This supplemental dataset also provides evidence that both OOPS-1 and SPE-11 are phosphoproteins. The mass spectra from the tandem-affinity purifications were analyzed for instances of prote..., , # Tandem-affinity purification of OOPS-1 and SPE-11 and identification of phosphorylation sites on OOPS-1 and SPE-11

Dataset DOI: [10.5061/dryad.931zcrjxk](10.5061/dryad.931zcrjxk)

## Description of the data and file structure

The data were collected as part of a study of early post-fertilization developmental mechanisms in the nematode *Caenorhabditis elegans* (Tsukamoto et al., 2025). In this study, it was found that an oocyte protein, OOPS-1, and a sperm protein, SPE-11, interact at fertilization, and that the complex is required for the completion of meiosis, the block to polyspermy, and eggshell formation. Tandem-affinity purification and mass spectrometry from *C. elegans* extracts showed that OOPS-1 and SPE-11 associate. This dataset presents the primary mass spectral evidence that OOPS-1 and SPE-11 associate. These data are analyzed in Table 3, Table S1, and Table S2 of the publication. These data are converted from the proprietary ThermoFisher .raw format to .mzML and a .txt...,