We evaluate native and engineered nitrogen fixation (nif) clusters from diverse sources transferred to cereal-associated bacteria to enable side-by-side comparisons of cluster performance and understa

NIAID Data Ecosystem30

pAM401-Based Shuttle Vectors That Enable Overexpression of Promoterless Genes and One-Step Purification of Tag Fusion Proteins Directly from Enterococcus faecalis

微生物基因工程

蛋白质纯化

Two novel Enterococcus faecalis-Escherichia coli shuttle vectors that utilize the promoter and ribosome binding site of bacA on the E. faecalis plasmid pPD1 were constructed. The vectors were named pM

PubMed Central10

G.oxydans 621H with a RhaSR-PrhaBAD expression system

微生物基因工程

合成生物学表达系统

G.oxydans 621H with plasmid pBBR1MCS-5-rhaSR-PrhaSR-PrhaBAD-mNG

NIAID Data Ecosystem40

Cloning and Expression of Three New Azotobacter vinelandii Genes Closely Related to a Previously Described Gene Family Encoding Mannuronan C-5-Epimerases

海藻多糖生物合成

微生物基因工程

The cloning and expression of a family of five modular-type mannuronan C-5-epimerase genes from Azotobacter vinelandii (algE1 to -5) has previously been reported. The corresponding proteins catalyze t

PubMed Central20

Seven inducible promoters for Zymomonas mobilis - MCF

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