Supplemental Tables S1-S3

Supplemental Table S1. The primer sequences utilized for quantitative real-time PCRF, forward primer; R, reverse primerSupplemental Table S2. Exogenous IL-6 preconditioning does not affect the hematological profile prior to DVT inductionOne day after rIL-6 or PBS administration, prior to DVT induction, whole blood was collected from the mouse heart, mixed with ethylenediaminetetraacetic acid (EDTA), and assessed for complete blood counts. Values for hemoglobin, leukocyte count, platelet count, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) are presented. Data are presented as mean ± standard error of the mean (SEM). Statistical analysis was performed using the unpaired t-test with Welch’s correction for all parameters, excluding MCV and MCHC, which were analyzed using the Mann-Whitney test; n = 5 mice per group.Supplemental Table S3. Bleeding time and coagulation cascade are not exerted by IL-6Bleeding time was assessed on the day of DVT harvest using the tail-vein filter paper method. The value for tail bleeding time is shown. Citrated plasma was collected on the day of DVT harvest for coagulation profile assay. Prothrombin time, activated partial thromboplastin time, and fibrinogen levels are shown. Data are presented as mean ± standard error of the mean (SEM). Statistical analysis was performed using the unpaired t-test with Welch’s correction for all parameters, excluding prothrombin time, using the Mann-Whitney test; n = 7-9 mice per group.