Transcriptomic analysis of the Csr system in Erwinia amylovora. Transcriptomic analysis of the Csr system in Erwinia amylovora

This RNA-seq analysis examined the global effect of CsrA and its non-coding small RNA (ncsRNA) csrB in E. amylovora under the T3SS-inducing condition. The csrA mutant showed differential expression of more than 20% genes in the genome, which include significant down-regulation of T3SS genes and those required for cell growth and viability. On the other hand, the csrB mutant exhibited significant up-regulation of most major virulence genes, suggesting antagonistic effect of csrB on CsrA targets. Overall design: Differential gene expression of the csrA and csrB mutants versus wild type grown in the T3SS-inducing condition for 6 hours