Single-cell RNA sequencing of sensory neurons in the mouse dorsal horn

We have used large-scale single-cell RNA sequencing (RNA-seq) to classify sensory neurons in the mouse dorsal horn, with the purpose of identifying neuronal subclasses, in particular making a systematic and comprehensive molecular classification of spinal cord sensory neurons, providing the neuronal basis for somatic sensation. Overall design: 1545 single cells from the mouse brain dorsal horn: 637 from strain C57/bl6, 537 from a vGat-cre-tdTomato line, and 371 from a vGlut2-eGFP line.