Nanostring mouse miRNA panel: performed on extracellular vesicles isolated following exposure to radiation

Plasma derived extracellular vesicles (EVs) have emerged as critical mediators of complications following traumatic injuries and after exposure to radiation. In response to injury, the cargo of these EVs is altered and can contain potent cargo that can influence cell function, including alterations in miRNA cargo. Here, we utilized NanoString's mouse miRNA panel to compare the alterations in miRNA content following exposure to 2 or 9 Gys of whole-body irradiation isolated 3 days after exposure and compared these changes to sham mice. Mice were exposed to either 2 or 9 Grays of whole-body irradiation and 3 days later the plasma EVs were isolated. These EVs were analyzed using NanoString's miRNA panel and the expression changes were compared to EVs isolated from sham mice.