A chromatin integration labeling method enables lower-input epigenomic profiling. A chromatin integration labeling method enables lower-input epigenomic profiling

We established a novel epigenomic profiling method ChIL-seq which does not employ immunoprecipitation, but instead exploits immunostaining. Overall design: We performed ChIL-seq on C2C12, NIH3T3, K562, MCF-7 and MDA-MB-231 cells with various cell numbers (1, 100, 1k, 10k and 100k cells) against various histone modifications and transcription factors, as well as H3K4me3 ChIP-seq for C2C12, MCF-7 and MDA-MB-231.