Comparative transcriptomic heat stress responses data for native Australian Acacia species: supplementary data and R code

To improve our understanding of heat tolerance across related species from diverse climates, we cultivated a selection of Acacia species in a controlled environment, using seed sourced from wild populations that occupy a wide range of Australian climates. After four months of growth in control temperature conditions (ca 24 °C day and 18 °C night) plants were exposed to a four-day heatwave (38 °C day and 26 °C night). Heatwave responses were quantified with coupled measurements of the transcriptome (gene expression) and PHT acclimation. Samples for transcriptomics were taken across three days: one day prior to heatwave (control), at the onset of heatwave conditions (Day 1) and at the end of the heatwave (Day 4). Accompanying measurements of PHT were taken the day prior and at day four using basal chlorophyll fluorescence.

This Collections includes RNA-seq metadata and cleaned expression data for data analysis (.rds file) and the associated Rmarkdown file (.Rmd file) to run analyses from our paper.