Expression data from developing hematopoietic stem and progenitor cells

In the mouse embryo during midgestation, hematopoietic stem cells (HSCs) are generated from aortic endothelium through the transdifferentiation process known as endothelial-to-hematopoietic transition, or EHT. During EHT, flat-shaped hemogenic endothelium transform into round-shaped hematopoietic cells, which subsequently form cell aggregates, called hematopoietic clusters. To understand HSC specification during ontogeny and to search for nascent HSC markers, we performed single-cell microarray analysis of developing HSC populations. Using cell sorter, we collected 85 single cells from 5 different populations: E10.5 endothelial cells, E10.5 hemogenic endothelial cells, E10.5 hematopoietic clusters, E12.5 hematopoietic clusters and E14.5 fetal liver HSCs.