Characterization of the role of SETDB1 in Muscle stem cells during skeletal muscle regeneration [scRNA-seq]

Muscle regeneration is process where different type of cells are interacting together to ensure a proper muscle regeneration. As Muscle stem cells are the central protagonist of muscle regeneration, we performed single cell RNA-seq to observe the change between the different cell types when SETDB1 is absent in Muscle stem cells. Cells were isolated from the conditional mice model Setdb1-cKO and were isolated by Fluorescence-activated cell sorting (FACS) using Hoeschst and Propidium iodide staining and analyzed using scRNAseq.