Primer sequences were obtained for the relevant genes of interest from GenBank (except for the primers for the circadian study arm).
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The primer sequences were analyzed for optimum primers using Primer 3 Software. Ready-to-use Quantitect Primer Assays (Qiagen) were purchased for the circadian arm of the study. Each assay contains forward and reverse primers that are generated from the NCBI Reference Sequence database, optimised and bioinformatically validated.
创建时间：
2015-12-02
