Developmental Programming: Impact of prenatal testosterone excess on liver and muscle transcriptome and the relationship between non-coding and coding RNA networks in female sheep [ncRNA-seq]

This study seeks to assess the effects of prenatal exposure of female sheep to execessive testosterone in metabolically relevant liver and muscle tissue. The goals are to 1) determine noncoding RNA in the control animals and 2) assess the effects of prenatal T-treatment on non-coding RNA. 3) Finally identify putative ncRNA-totalRNA interactions. Overall design: 8 Female Sheep. 4 Controls and 4 Treatment. 2 Tissues-Liver and Muscle. 16 Samples total- 8 Samples Liver(4C, 4T) 8 Samples Muscle(4C, 4T)