Gene expression in the isolated islets of 10 day old, 4 wk old, 12 wk old, and 20 wk old Non-Obese Diabetic (NOD) mice compared to healthy age matched control NOD.B10 mice

Gene expression in the islets of diseased NOD mice compared to congenic healthy NOD.B10 mice at various ages, during the progression of NOD disease. Islets were isolated from individual NOD and NOD.B10 mice by collagenase digestion. RNA was extracted using Trizol, combined with the RNeasy micro kit (Qiagen). RNA quality was assessed using the Agilent 2100 Bioanalyzer and the RNA 6000 Pico Reagent Kit (Agilent). Microarrays were performed using the Whole Mouse Genome Microarray Kit, 4x44K 2-color arrays (Agilent Technologies).在NOD疾病进展过程中，对不同年龄阶段的患病NOD小鼠胰岛与同类系健康NOD.B10小鼠的胰岛基因表达情况进行对比分析。研究人员通过胶原酶消化法，从单只NOD小鼠与NOD.B10小鼠体内分离得到胰岛。采用Trizol试剂结合RNeasy微量试剂盒（Qiagen）提取RNA。使用安捷伦2100生物分析仪（Agilent 2100 Bioanalyzer）及RNA 6000 Pico试剂试剂盒（Agilent）对RNA质量进行评估。采用安捷伦科技（Agilent Technologies）的全小鼠基因组微阵列试剂盒（4x44K双色微阵列）完成微阵列实验。