MALDI-TOF MS Main Spectra Profile (MSP) of Paenibacillus taichungensis Lee et al. 2008 DSM 19942ᵀ

This dataset presents a MALDI-TOF MS Main Spectra Profile (MSP) generated for the type strain of Paenibacillus taichungensis Lee et al. 2008 DSM 19942ᵀ As a type strain, this isolate represents the taxonomic reference for the species. However, at the time of data acquisition, no corresponding MSP was available in the Bruker MALDI Biotyper reference library. The dataset provides a high-quality reference protein mass spectral profile intended to support microbial identification, database expansion, and improved taxonomic resolution within the genus Paenibacillus. By providing an experimentally validated MSP for a type strain, this dataset contributes to bridging the gap between formal taxonomic description and routine MALDI-TOF MS-based identification workflows. MALDI-TOF MS analyses and MSP generation were performed using a Bruker MALDI-TOF MS platform following standard Bruker Biotyper protocols recommended by the manufacturer for bacterial identification and taxonomic studies. Spectral acquisition, preprocessing, quality control, and MSP generation were conducted using default parameters, without non-standard or experimental modifications. Sample Preparation and Spectra Acquisition P. taichungensis DSM 19942ᵀ was cultured on TSA plates at 30 °C for 48 h. Protein extraction was performed using the standard ethanol/formic acid (EtOH/FA) protocol recommended by the MALDI Biotyper manufacturer (Bruker Daltonics). Spectra were acquired in linear positive ion mode within a mass range of 2,000–20,000 m/z using a Microflex LT/SH instrument controlled by flexControl 3.4 software (Bruker Daltonics). External calibration was performed using the Bruker Bacterial Test Standard according to the manufacturer’s instructions. At least eight technical replicates were included for MSP generation. Raw spectra were processed using default preprocessing parameters in flexAnalysis 3.4. The Main Spectrum Profile (MSP) was generated using the automated function implemented in MALDI Biotyper Compass Explorer Module 4.1.80 (Bruker Daltonics). All spectra were visually inspected for quality prior to MSP creation. Strain information The type strain of Paenibacillus taichungensis Lee et al. 2008 DSM 19942ᵀ was obtained from the Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures DSMZ. -----------------------Author contributions Data acquisition, sample preparation, and MALDI-TOF MS analysis (including MSP generation) were performed by Anna-Karina Kaczorowska. This work was conducted within a research project entitled “Let’s dry up and survive together: is anhydrobiosis in water bears (tardigrades) modulated by a specific microbiome community, and does it depend on bacteria that survive desiccation together with them?”, led by Monika Mioduchowska.