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Specific activities and kinetic parameters of the wild type FClip1 and the deleted mutants.

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Specific activities were measured in 50 mM phosphate buffer (pH 8.0) at 75°C using pNPC4 and pNPC12 as the substrates, respectively. Kinetic parameters were obtained in 50 mM phosphate buffer (pH 8.0) at 75°C using pNPC4 as the substrate. The fitting curves for kinetic parameters are presented in Figure S6. aNumbers in brackets indicate the values relative to wild type.

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2012-10-08
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