Type I and II IFN Conditioning of Human Macrophage Gene Expression Responses

Macrophage responses to pathogen-associated molecules are modulated by prior conditioning with cytokines such as interferons (IFNs). We conducted RNA-seq studies characterizing how conditioning with Type I or II IFN alters the gene expression programs of human macrophages responding to TLR ligands, TNF, and IFN??. Employing a sequential conditioning and stimulation approach, we found that IFN?? and IFN?? have partially overlapping but also remarkably distinct effects.