We co-cultured T cells with Mo-DCs exposed to ConA for 24 h and used multiplex PCR and next-generation sequencing to detect the TCRa repertoire.The length of the amino acids of CDR3 in each group was
adetermined using consensus results from IMGT/V-QUEST and IgBlast.bThe top D gene matches determined by both IMGT/V-QUEST and IgBlast are included.If there were multiple matches, all of the top matche
Determing the individual heritable differences result in unique B and T cell receptor repertoires of naive and antigen-experienced cells through next gerneration sequencing.
Immunology Database and Analysis Portal2025-10-30 更新40
We longitudinally sequenced the repertoires of six South African donors 1) prior to infection and through 2) acute and 3) chronic HIV-1 infection and quantified how the antibody repertoire changed ove