Physiological responses of A. flavipes to S starvation.

Spores of A. flavipes were starved of sulfur (cultured on Dox’s medium with no S compounds) or non-starved control (cultured on Dox’s liquid medium with 5 mM MgSO4) for 24 h, then inoculated to Dox’s liquid medium amended with MgSO4, L-Methionine, L-Cysteine, L-Cystine and Glutathione.–S control represents culture on Dox’s medium with no sulfur compounds. Fresh weight (A) and concentration of total intracellular proteins (B), extracellular proteins (C), and total glutathione (D) were determined after fives day of culture. Data shown are means ± SD of three replications.