Gene expression profiles at the single cell levels of murine tumor inflitrating PD-1 and PD-1 KO NK cells

Here, we found by single cell RNA sequencing analysis that PD-1 can be expressed on MHC class I-deficient tumor-infiltrating NK cells in vivo. We also demonstrate distinct alterations in the phenotype of PD-1-deficient NK cells and a more mature phenotype which might reduce their capacity to migrate and kill in vivo. Overall design: Using WT and PD-1-/- mice, we performed single cell RNA-sequencing (scRNA-SEQ) using the SMART-SEQ2 platformon tumor-infiltrating NK cells from mice inoculated with the MTAP1A tumor.