Stability and diversity of the urinary microbiota

When comparing studies it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition.For comparison of different storage conditions, urine was collected from five healthy adult female donors, exposed to different storage conditions, and analyzed by 16S rRNA gene sequencing. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in nineteen healthy donors, including four women, five men, five girls, and five boys.