De novo design of high-affinity HER2-targeting protein minibinders

This dataset contains the original, unprocessed data supporting the study on de novo designed minibinders targeting HER2 domain IV. The data include raw files from flow cytometry, isothermal titration calorimetry (ITC), circular dichroism (CD) spectroscopy, and confocal fluorescence microscopy. These files provide direct experimental evidence for minibinder binding activity, protein stability, and cellular localization.本数据集包含支撑靶向人表皮生长因子受体2（HER2）IV结构域的从头设计迷你结合蛋白（minibinders）相关研究的原始未处理数据。该数据集涵盖流式细胞术、等温滴定量热法（ITC）、圆二色谱（CD）光谱法以及共聚焦荧光显微镜成像的原始文件，可为迷你结合蛋白的结合活性、蛋白质稳定性及细胞定位提供直接的实验证据。