Validation of transcript expression of L. (V.) panamensis-infected human monocyte-derived macrophages.

Comparison of transcript expression in human macrophages infected with L. (Viannia) panamensis by quantitative real time PCR versus microarray analyses. Relative expression is expressed as a ratio of transcripts in infected cells over uninfected cells at 30 minutes or 4 hours after infection/interaction was determined. The values presented for the quantitative reverse transcriptase real time PCR analyses are the geometric mean of the ratios of macrophage transcripts from 6 to 7 different donors. The gene expression at each time point was normalized by the geometric mean of GAPDH and 39 S ribosomal protein L18 [36]. The genes listed are: IL1-β – interleukin-1β, TNFα – tumor necrosis factor-α, GMCSF2 –colony-stimulating factor 2 (granulocyte-macrophage), PTGS2 – prostaglandin-endoperoxide synthase 2, and IL6 – interleukin 6. The 95%CI are indicated between brackets.