Transcription profiling by array of mouse embryos with Cited2 knock-out mutation
收藏Alliance of Genome Resources2026-08-01 收录
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Total RNA (15 ug) isolated from three each of wild type and Cited2-null E14.5 fetal livers using Qiagen RNeasy kit (Valencia, CA) was used to prepare biotinylated cRNA according to the protocol described in Affymetrix Expression Analysis Technical Manual. We hybridized a total of six GeneChip Mouse Genome 430A 2.0 arrays (Affymetrix), three for controls and three for experimental samples.
