Data for: Microbial community structure within a weathered waste-rock pile overlain by a monolayer soil cover

A historic waste-rock stockpile at the Detour Lake Mine, covered with a thin layer of local overburden, was studied to determine the potential for microbially-mediated generation of acid rock drainage. The attached Microbiology file contains detailed research data describing: (a) enumerations of acidophilic sulfur- (SOMa) and iron-oxidizing microorganisms (IOMa), using the "most probable number" (MPN) technique (tab "MPN raw data"); (b) relative abundances (as % of total reads) of fungi, using high-throughput amplicon sequencing of 18S rRNA genes (tabs "fungi family" and "fungi genus"); (c) relative abundances (as % of total reads) of bacteria, using high-throughput amplicon sequencing of 16S rRNA genes (tabs "bacteria family", "bacteria genus", and "bacteria genus by type").本研究针对Detour Lake矿（Detour Lake Mine）一处历史堆存的废石堆展开调研，该废石堆表面覆盖有薄层当地覆盖层，旨在探究微生物介导产生酸性岩石排水（acid rock drainage）的潜力。随附的微生物学文件包含如下详细研究数据：(a) 采用“最大似然数”（most probable number, MPN）技术，对嗜酸硫氧化微生物（acidophilic sulfur-oxidizing microorganisms, SOMa）与铁氧化微生物（iron-oxidizing microorganisms, IOMa）进行计数，相关原始数据位于工作表"MPN raw data"；(b) 通过对18S rRNA基因进行高通量扩增子测序，分析真菌的相对丰度（以总测序读段数的百分比计），相关数据分别位于工作表"fungi family"与"fungi genus"；(c) 通过对16S rRNA基因进行高通量扩增子测序，分析细菌的相对丰度（以总测序读段数的百分比计），相关数据分别位于工作表"bacteria family"、"bacteria genus"以及"bacteria genus by type"。