Examples of <i>k</i>_<i>cat</i> values from BRENDA and the minimal values required to produce the reaction fluxes measured in ¹³C fluxomics experiments.

The minimal kcat is obtained by dividing 13C fluxes by mean protein counts using the Gene-Protein-Reaction (GPR) rules. The final kcat is obtained by the doubling procedure to reproduce a mean growth rate of 0.35 hr−1, and the final values of the associated mean vmax values obtained by taking mean vmax over 1000 cells from a population. Factor of 3.0 × 10−7 converts the units from cell−1 s−1 to mmol gDwt−1 hr−1. When more than one enzyme is responsible for a reaction, the GPR rules are used to determine how to process protein counts (sum of subunits in case of ‘or’ and minimum count among the subunits for ‘and’).