Next Generation Sequencing of OVA-treated MutuDC Transcriptome

The goal of this study is to get the profile of the OVA-treated MutuDC transcriptome. Based on the dada of transcriptome, we processed our sgRNA sequencing data of genome-wide CRISPR/Cas9 screening. Briefly, after we have obtained the enriched gene list from our sgRNA sequencing, we excluded the genes undetected in OVA-treated MutuDC transcriptome to further refine our screening gene list. Overall design: To get the profile of the OVA-treated MutuDC transcriptome, we did the next generation sequencing of MutuDC at 9 h after treated with 3 mg/ml OVA.